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Nature提出新概念:如何获得高质量的抗体
  来自世界各地的100多名研究人员共同发出了一项倡议,这有可能从根本上改变鉴定研究用抗体的方式。相关文章发表在2月5日的《自然》(Nature)杂志上。

Nature提出新概念:如何获得高质量的抗体

Nature提出新概念:如何获得高质量的抗体


来自世界各地的100多名研究人员共同发出了一项倡议,这有可能从根本上改变鉴定研究用抗体的方式。相关文章发表在2月5日的《自然》(Nature)杂志上。


洛斯阿拉莫斯国家实验室(Los Alamos National Laboratory)研究员Andrew Bradbury说:“我们提出,就像基因一样,抗体也应由它们的序列来定义,并且它们应该是在细胞系中重组生成。”


根据编码各种亚基的序列来参考每次检测所用的抗体,它们的浓度和标准化实验缓冲液,可以让全世界的研究人员能够在相同条件下使用具有相同亲和力的抗体。


一种抗体或结合试剂的序列,是该试剂的最终“条形码”,其确保了每个人都能够使用相同的试剂来检测同一目标。导出这一条形码涉及到从体外文库中挑选出一些抗体,或是克隆及测序来自杂交瘤的抗体基因等工作。这将要求对抗体供应的模式作出重大的改变。


抗体是可以帮助机体识别与中和细菌、以及对免疫系统发起的其他攻击的一类特殊蛋白质,科学家们一直利用它们来作为特异的结合试剂。抗体质量控制和精确识别是那些有着序列测定和重组表达需求的研究者们一直努力寻求解决的问题(延伸阅读:基因序列能被快速测定和配对 新技术高效生产"高亲和"纳米抗体 )。Bradbury指出,不同于基因、寡核苷酸、质粒、重组蛋白等,抗体是生物学研究中唯一没有在序列水平上进行定义的广泛应用试剂。

Nature提出新概念:如何获得高质量的抗体

Nature提出新概念:如何获得高质量的抗体


研究人员注意到,所有抗体的质量因制造商不同而存在巨大的差异,大多数抗体很少进行验证,批次间差异极为常见。
并且,批量产品附带的证明文件质量同样差异巨大;甚至提供的证明文件往往并不对应提供的批次。


“为了杜绝由于缺乏验证和鉴定所造成的对材料、研究人员时间以及金钱的巨大浪费,必须要根据它们的序列来定义抗体,并且要在标准条件下进行重新生产,”作者们指出。这里的“重新”生产是指采用一些不涉及动物免疫的方法来从头生成抗体或其他结合试剂,而非基于免疫接种生成的克隆抗体。


例如,多克隆抗体的生产是通过将一种蛋白质注射到动物体内,然后提取出响应这一免疫蛋白动物血液中生成并携带着的抗体。各种各样的抗体并非高精度,只有0.5-5%的产物是想得到的抗体,可对初始目标产生反应。其余的都是原本存在于动物血流中的抗体,表明了动物以往的免疫应激。漫无目的地收获如此多错误的抗体,使得生成了一些特异性较差的抗体,由此造成了研究领域的浪费。


“我们建议,借助一种体外方法来生成抗体,这样根本不需要使用动物,重要的是还将直接生成具有已知序列的分子,”Bradbury说。

共 140 个关于Nature提出新概念:如何获得高质量的抗体的回复 最后回复于 2017-4-9 19:14

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发表于 2017-1-22 02:53:06 | 显示全部楼层

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发表于 2017-1-22 02:55:35 | 显示全部楼层

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发表于 2017-1-22 03:04:27 | 显示全部楼层

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发表于 2017-1-23 15:58:49 | 显示全部楼层

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